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COMPANION ANIMALS

RIDX®

CCV/CPV Ag Combo Test Kit
[CGM-CCG-21]

INQUIRY CAT IFU

DESCRIPTION

Canine coronavirus (CCV) and canine parvovirus (CPV) are the two main causes of diarrhea disease in dogs.
The RIDX® CCV/CPV Ag Combo Test Kit is a lateral flow chromatographic immunoassay for the qualitative detection of CCV and CPV antigens in canine feces. This kit shows two letters which are the test (T) line and the control (C) line for each test on the surface of the device. If viral (CCV or CPV) antigens exist in the sample, that bind to the gold-conjugated virus (CCV or CPV) specific antibody. The antigen-antibody complex moves through the membrane by capillary force and responds to the virus (CCV or CPV) specific antibody on the test line, resulting in a red line. The control line indicates that the test is performed correctly and should appear when the test is complete.
The highly selective and sensitive monoclonal antibody to CCV (or CPV) is used as capture and detector in the kit. The RIDX® CCV/CPV Ag Combo Test Kit can detect CCV antigens and CPV antigens in canine feces with high accuracy.

SPECIMEN

Canine feces

COMPONENTS

• CCV/CPV Ag Combo test device (10 tests)
• Sample dilution buffer (10 vials)
• Disposable swab (10 ea)
• Disposable dropper (10 ea)
• Instructions for use (1 sheet)

FEATURES

• Sensitivity: CCV 94.9% (56/59) vs. RT-PCR / CPV 98.1% (101/103) vs. PCR
• Specificity: CCV 97.5% (118/121) vs. RT-PCR / CPV 100% (214/214) vs. PCR
• Diagnostic Agreement: CCV 96.6% (174/180) vs. RT-PCR / CPV 99.3% (315/317) vs. PCR
• Limit of Detection: CCV 1x105 TCID50/mL / CPV 5x103 TCID50/mL
• No cross-reactivity with other canine pathogens (CDV, CIV, Escherichia coli, Giardia cyst, and Salmonella spp.)

PACKAGE

• 10 Tests/Kit

TEST PROCEDURE

1. All reagents and samples must be at room temperature (15~30℃/59~86℉) before use.
2. Collect feces samples using a swab.
3. Put the swab into the sample dilution buffer and stir the solution with the swab to disperse the sample into the buffer (approximately 10 seconds).
4. Remove the swab from the sample dilution buffer.
5. Wait for 20 seconds to settle down the large particles.
6. Remove the test device from the pouch and place it on a flat and dry surface.
7. Take the supernatant sample in the tube by using a disposable dropper.
8. Add 4 drops of the mixed sample into each sample holes (S), drop by drop vertically.
9. Read test results at 10 minutes.

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