The RIDX^® Bovine Pregnancy Test Kit is a lateral flow chromatographic immunoassay that detects early PAGs (Pregnancy-Associated Glycoproteins) contained in bovine whole blood, serum, and plasma. Since 1970s, the PAG is widely recognized as one of the most efficient markers for detecting pregnancy in cattle.
If PAG is present in the sample, it binds to the PAG-specific antibody–nanoparticle conjugate. A red line appears at the location of the test line due to binding to the PAG-specific antibody that moves through the membrane by capillary action and is dispensed on the membrane. If PAG is not present in the sample, no reaction occurs on the test line and a red line appears only on the control line.
The highly selective and sensitive two monoclonal antibodies to PAG are used as capture and detector in the kit. This kit detects PAG quickly and simply within 20 minutes after applying the sample. The RIDX^® Bovine Pregnancy Test Kit is a diagnostic reagent for pregnancy of cattle with high accuracy.

Bovine blood (whole blood, serum or plasma)

• RIDX Bovine Pregnancy test kit (25 or 2 strips)
• Dilution Buffer (1 vial)
• Disposable dropper for whole blood sample (25 or 2 ea)
• Test tube (25 or 2 ea)
• Instructions for use (1 sheet)

• Sensitivity: 99.16% (354/357) vs. ELISA
• Specificity: 97.01% (195/201) vs. ELISA
• Diagnostic Agreement: 98.39% (549/558) vs. ELISA
• Limits of Detection: 0.3 ng/mL of PAG
• No cross-reactivity with Progesterone and Early Conception Factor (also known as Early Pregnancy Factor) of cattle.

• 25 Tests/Kit [LGM-BPG-11]
• 2 Tests/Kit [LGM-BPG-12]

[Whole blood]

1. All samples and test components should be at room temperature (15~30℃/59~86℉) before use.
2. Transfer 3 drops (approximately 100 μL) of the sample dilution buffer to a test tube.
3. Take 40 μL of the anticoagulated whole blood using a disposable dropper unto the black line on the dropper and instill it onto the green line of the sample pad on the PAG strip. When instilling anticoagulated whole blood onto the sample pad of the strip, be sure to use the provided disposable dropper.
4. After all the whole blood has been absorbed into the sample pad (approximately 1~2 minutes), place the PAG strip into the test tube containing the sample dilution buffer.
5. Read test results at 20 minutes.

[Serum or plasma]

1. All samples and test components should be at room temperature (15~30℃/59~86℉) before use.
2. Using a micropipette, transfer 150 μL of serum or plasma into a test tube.
3. Place the PAG strip into the test tube containing the serum or plasma sample.
4. Read test results at 20 minutes.