Newcastle disease virus (NDV), belongs to the genus Avulavirus in the family Paramyxoviridae and is the causative agent of Newcastle Disease (ND), a highly contagious disease that primarily affects avian species, particularly domestic poultry. Economic losses are exacerbated by sudden drops in egg production and the production of abnormal eggs.
The RIDX^® NDV Ag Test Kit is a lateral flow chromatographic immuno-assay for the qualitative detection of NDV in poultry. This kit shows two letters which are the test (T) line and the control (C) line on the surface of the device. If the NDV antigen exists in the sample, it binds to the gold-conjugated anti-NDV antibody. The antigen-antibody complex moves through the membrane by capillary force and responds to the secondary anti-NDV antibody on the test line, resulting in a red line. The control line indicates that the test is performed correctly and should appear when the test is complete.
Two different monoclonal antibodies to the haemagglutinin-neuraminidase (HN) glycoprotein of NDV are used as a capture and detector in the kit. The RIDX^® NDV Ag Test Kit can detect NDV in poultry trachea, spleen, or kidney swabs, or these tissue homogenates with high accuracy.

Avian trachea, spleen or kidney

• RIDX NDV Ag test device (10 tests)
• Sample dilution buffer (10 tubes)
• Disposable swab (10 ea)
• Dropper cap with filter (10 ea)
• Paper rack for standing buffer tubes (1 ea)
• Instructions for use (1 sheet)

• Sensitivity: 88.24% (15/17) vs. RT-PCR
• Specificity: 100% (16/16) vs. RT-PCR
• Diagnostic Agreement: 93.94% (31/33) vs. RT-PCR
• Limits of Detection: 1x10^4.3 EID₅₀/mL
• No cross-reactivity with other avian pathogens (AIV, IBDV, IBV, Mycoplasma gallisepticum, and Mycoplasma synoviae)

• 10 Tests/Kit [LGM-YNG-11]

1. All samples and test components should be at room temperature (15~30°C/59~86℉) before use.
2. Using a swab to collect specimen.
3. Put the swab into the sample dilution buffer tube and stir the solution 10 times with the swab to disperse the specimen into the buffer.
4. Break the head of the cotton swab and discard the rod.
5. Attach a dropper cap to the top of the buffer tube.
6. Apply 4 drops (approximately 100 μL) of the processed solution in the sample hole on the device.
7. Read test result at 10 minutes.