Infectious bronchitis virus (IBV) belongs to the genus Coronavirus and is the causative agent of infectious bronchitis (IB), an acute and highly contagious viral disease of chickens. IBV is one of the most damaging pathogens in the poultry industry due to significant losses from reduced growth, decreased egg production, poor egg quality, and biosecurity costs.
The RIDX^® IBV Ag Test Kit is a lateral flow chromatographic immuno-assay for the qualitative detection of IBV in poultry.
This kit shows two letters which are the test (T) line and the control (C) line on the surface of the device. If the IBV antigen exists in the sample, it binds to the gold-conjugated anti-IBV antibody. The antigen-antibody complex moves through the membrane by capillary force and responds to the secondary anti-IBV antibody on the test line, resulting in a red line. The control line indicates that the test is performed correctly and should appear when the test is complete.
Two different monoclonal antibodies to the spike (S) glycoprotein of IBV and two different monoclonal antibodies to the QX strain of IBV are used as captures and detectors in the kit. The RIDX^® IBV Ag Test Kit can detect IBV in poultry fecal, cloacal or tracheal swabs or tissue (kidney or trachea) homogenates with high accuracy.

Avian feces, cloaca, trachea, or kidney

• RIDX IBV Ag test device (10 tests)
• Sample dilution buffer (10 tubes)
• Disposable swab (10 ea)
• Dropper cap with filter (10 ea)
• Paper rack for standing buffer tubes (1 ea)
• Instructions for use (1 sheet)

• Sensitivity: 87.50% (14/16) vs. RT-PCR
• Specificity: 100% (16/16) vs. RT-PCR
• Diagnostic Agreement: 93.75% (30/32) vs. RT-PCR
• Limits of Detection: 5x10^4.8 EID₅₀/mL
• No cross-reactivity with other avian pathogens (AIV, IBDV, NDV, Mycoplasma gallisepticum, and Mycoplasma synoviae)

• 10 Tests/Kit [LGM-YBG-11]

1. All samples and test components should be at room temperature (15~30°C/59~86℉) before use.
2. Using a swab to collect specimen.
3. Put the swab into the sample dilution buffer tube and stir the solution 10 times with the swab to disperse the specimen into the buffer.
4. Break the head of the cotton swab and discard the rod.
5. Attach a dropper cap to the top of the buffer tube.
6. Apply 4 drops (approximately 100 μL) of the processed solution in the sample hole on the device.
7. Read test result at 10 minutes.