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AIV Ag Test Kit
[LGM-YAG-01] & [LGM-YAG-02]
Avian influenza refers to the disease caused by infection with avian influenza Type A viruses. These viruses occur naturally among wild aquatic birds worldwide and can infect domestic poultry and other bird and animal species.
The RIDX® AIV Ag Test Kit is a lateral flow chromatographic immunoassay for the qualitative detection of avian influenza virus (AIV) antigens in poultry specimen. This kit shows two letters which are the test (T) line and the control (C) line on the surface of the device. If the AIV antigen exists in the specimen, it binds to the cellulose nanobeads-conjugated AIV antibody. The antigen-antibody complex moves through the membrane by capillary force and responds to the AIV antibody on the test line, resulting in a red line. The control line indicates that the test is performed correctly and should appear when the test is complete.
Two monoclonal antibodies to the conservative AIV nucleocapsid protein (NP) are used as capture and detector in the kit. The RIDX® AIV Ag Test Kit can detect various subtypes of AIV NPs in poultry fecal, cloacal, or oropharyngeal swabs, or tissue (trachea or lung) homogenate swabs with high accuracy at the farm field.
Avian feces, cloaca, oropharynx, trachea, or lung
• RIDX AIV Ag test device (30 or 10 tests, 10 tests x 3 sets or 10 tests x 1 set)
• Sample dilution buffer (30 or 10 tubes)
• Disposable swab (30 or 10 ea)
• Dropper cap with sample filter (30 or 10 ea)
• Paper rack for standing buffer tubes (1 ea)
• Instructions for use (1 sheet)
• Sensitivity: 100% (97/97) vs. RT-PCR
• Specificity: 99.5% (206/207) vs. RT-PCR
• Diagnostic Agreement: 99.6% (303/304) vs. RT-PCR
• Limit of Detection: 5x103.0 TCID50/mL (H5N1), 1x104.3 EID50/mL (H5N2), 0.5x103.0 EID50/mL (H5N8 & H5N6)
• No cross-reactivity with other avian pathogens (IBDV, IBV, ILTV, Mycoplasma gallisepticum, and NDV)
• 30 Tests/Kit [LGM-YAG-01]
• 10 Tests/Kit [LGM-YAG-02]
1. All test components and specimens must be at room temperature (15~30℃/59~86℉) before use.
2. Using a swab to collect specimen.
3. Put the swab into the sample dilution buffer and stir the solution 10 times with the swab to disperse the specimen into the buffer.
4. Break the head of the cotton swab and discard the rod.
5. Attach a dropper cap with a filter to the top of the buffer tube.
6. Apply 4 drops of the processed solution in the sample hole on the device.
7. Read test result at 15 minutes.