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LIVESTOCK ANIMALS

RIDX®

BVDV Ag SP Test Kit
[LGM-BVG-12]

INQUIRY CAT IFU

DESCRIPTION

Bovine viral diarrhea virus (BVDV) is a highly contagious pathogen that causes significant economic losses around the world due to its impact on reproduction, respiratory health, and immunosuppression in cattle and other ruminants.
The RIDX® BVDV Ag SP Test Kit is a lateral flow chromatographic immunoassay for the qualitative detection of BVDV in bovine serum or plasma. This kit shows two letters which are the test (T) line and the control (C) line on the surface of the device. If the BVDV antigen exists in the sample, it binds to the gold-conjugated anti-BVDV antibody. The antigen-antibody complex moves through the membrane by capillary force and responds to the secondary anti-BVDV antibody on the test line, resulting in a red line. The control line indicates that the test is performed correctly and should appear when the test is complete.
Two different monoclonal antibodies against the recombinant conservative nonstructural NS3 protein of BVDV are used as a capture and detector in the kit. The RIDX® BVDV Ag SP Test Kit can detect BVDV in bovine serum or plasma with high accuracy

SPECIMEN

Bovine serum or plasma

COMPONENTS

• RIDX BVDV Ag test device (10 tests)
• Dilution buffer (1 vial)
• Disposable dropper (10 ea)
• Instructions for use (1 sheet)

FEATURES

• Sensitivity: 96.67% (58/90) vs. RT-PCR
• Specificity: 100% (92/92) vs. RT-PCR
• Diagnostic Agreement: 98.68% (150/152) vs. RT-PCR
• Limits of Detection: 1x104 TCID50/mL
• No cross-reactivity with other bovine pathogens (Aino virus, Akabane virus, BCV, Rotavirus, CHUV and IBAV)

PACKAGE

• 10 Tests/Kit [LGM-BVG-12]

TEST PROCEDURE

1. All samples and test components should be at room temperature (15~30°C/59~86℉) before use.
2. Take 50 μL of sample to the black line of the dropper.
3. Apply the taken sample into the sample hole (S).
4. Apply 3 drops (approximately 100 μL) of the dilution buffer into the sample hole (S).
5. Read test result at 20 minutes.

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