Feline panleukopenia virus (FPV, also known as feline parvovirus) and feline coronavirus (FCoV) are contagious pathogens responsible for acute gastroenteritis in cats. Despite structural, genetic, molecular biological, and pathological differences, initial clinical signs of two viruses, including diarrhea, vomiting, fever, lethargy, and anorexia, are very similar when infected with cats.
The RIDX^® FPV/FCoV Ag Combo Test Kit is a lateral flow chromatographic immunoassay for the qualitative detection of FPV antigens and FCoV antigens in feline feces. This kit shows two letters which are the test (T) line and the control (C) line for each test on the surface of the device. If viral antigens exist in the sample, that bind to the gold-conjugated virus (FPV or FCoV) specific antibody. The antigen-antibody complex moves through the membrane by capillary force and responds to the virus (FPV or FCoV) specific antibody on the test line, resulting in a red line. The control line indicates that the test is performed correctly and should appear when the test is complete.
The highly selective and sensitive two monoclonal antibodies to FPV or FCoV for each are used as capture and detector in the kit. The RIDX^® FPV/FCoV Ag Combo Test Kit can detect FPV antigens and FCoV antigens in feline feces with high accuracy.

Feline feces

• FPV/FCoV Ag Combo test device (10 tests)
• Sample dilution buffer (10 vials)
• Disposable swab (10 ea)
• Disposable dropper (10 ea)
• Instructions for use (1 sheet)

• Sensitivity: FPV 97.4% (76/78) vs. PCR / FCoV 92.8% (13/14) vs. RT-PCR
• Specificity: FPV 98.4% (123/125) vs. PCR / FCoV 98.1% (52/53) vs. RT-PCR
• Diagnostic Agreement: FPV 98.0% (199/203) vs. PCR / FCoV 97.0% (65/67) vs. RT-PCR
• Limit of Detection: FPV 1x10^5.5 TCID₅₀/mL / FCoV 1.97x10⁴ TCID₅₀/mL
• No cross-reactivity: FPV against FCV, FCoV, FeLV, E. coli, Giardia, and Salmonella / FCoV against FCV, FeLV, FPV, E. coli, Giardia, and Salmonella

• 10 Tests/Kit

1. All reagents and samples must be at room temperature (15~30℃/59~86℉) before use.
2. Collect feces samples using a swab.
3. Put the swab into the sample dilution buffer and stir the solution with the swab to disperse the sample into the buffer (approximately 10 seconds).
4. Remove the swab from the sample dilution buffer.
5. Wait for 20 seconds to settle down the large particles.
6. Remove the test device from the pouch and place it on a flat and dry surface.
7. Take the supernatant sample in the tube by using a disposable dropper.
8. Add 4 drops of the mixed sample into each sample holes (S), drop by drop vertically.
9. Read test results at 10 minutes.